Trypan blue as a slow migrating dye for SSCP detection in polyacrylamide gel electrophoresis.

2.Cusi, M.G., M. Valassina and P.E. Valensin. 1994. Comparison of M-MLV reverse transcriptase and Tth polymerase activity in RTPCR of samples with low virus burden. BioTechniques 17:1034-1036. 3.Fiorenza, M.T. and F. Mangia. 1998. Quantitative RT-PCR amplification of RNA in single mouse oocytes and preimplantation embryos. BioTechniques 24:618-623. 4.Huang, Z., M.J. Fasco and L.S. Kaminsky. 1996. Optimization of DNase I removal of contaminating DNA from RNA for use in quantitative RNA-PCR. BioTechniques 20:1012-1020. 5.Kunitz, M. 1950. Crystalline desoxyribonuclease: I. Isolation and general propertiesSpectrophotometric method for the measurement of deoxyribonuclease activity. J. Gen. Physiol. 33:349-362. 6.Mallet, F., G. Oriol, C. Mary, B. Verrier and B. Mandrand. 1995. Continuous RT-PCR using AMV-RT and Taq DNA polymerase: characterization and comparison to uncoupled procedures. BioTechniques 18:678-687. 7.Rand, K.H. and H. Houck. 1990. Taq polymerase contains bacterial DNA of unknown origin. Mol. Cell. Probes 4:445-450. 8.Rochelle, P.A., A.J. Weightman and J.C. Fry. 1992. DNase I treatment of Taq DNA polymerase for complete PCR decontamination. BioTechniques 13:520. 9.Schmidt, T.M., B. Pace and N.R. Pace. 1991. Detection of DNA contamination in Taq polymerase. BioTechniques 11:176-177. 10.Zimmerman, S.B. and N.F. Coleman. 1971. Pancreatic deoxyribonuclease-The role of dimerization in the reversible thermal inactivation at acid pH. J. Biol. Chem. 246:309-317.